Intestinal genetic inactivation of caspase-8 diminishes migration of enterocytes

Figure 1 Migration of caspase-8 (Casp8)-deleted Caco2 cells is diminished. A: Western blots Casp8 protein and densitometric analysis after treatment of Caco2 cells with small interfering RNAs (siRNAs) interfering with Casp8 transcripts or with negative control siRNA (∆scramble); B: Measurement of cellular proliferation by proliferating cell nuclear antigen (PCNA) expression in Caco2 cells with siRNA interfering with Casp8 transcripts or with negative control siRNA; C: Migration of Casp8 siRNA-treated Caco2 cells and scramble controls at 6 h and 24 h after scratching; D-F: Control siRNA-treated Caco2 cells at 0 h (D), 6 h (E), or 24 h (F) after scratching; G-I: Casp8 siRNA-treated Caco2 cells at 0 h (G), 6 h (H) or 24 h (I) after scratching.