Degradation of intestinal mRNA: A matter of treatment

Figure 5 Measurement of RNA Integrity in different parts of the gut. Rat gut was separated in different parts (proximal small intestine, distal small intestine, caecum and colon with rectum) and then the samples were stored in medium at room temperature and at different points in time (0 h, 8 h, 24 h and 48 h) the tissue was separated in smooth muscle (muscle) and submucosal/mucosal layer (mucosa). The RNA Integrity numbers were calculated and data presented as the mean ± SE and tested for statistical significance using ANOVA. The results were considered significant with P < 0.001 and n = 9.