Primary analysis and screening of microRNAs in gastric cancer side population cells

Table 1 MicroRNA microarray and quantitative real-time-polymerase chain reaction analyses results

Down-regulated genes	Fold change microarray	ΔCT	-ΔΔCT	Upregulated genes	Fold change microarray	ΔCT	-ΔΔCT
		qRT-PCR				qRT-PCR
hsa-miR-3175	7.29	21.82	-1.32	hsa-miR-25-star	27.35	6.63	4.47
hsa-miR-1246	3.46	14.07	-0.64	hsa-miR-1275	5.13	13.77	0.48
hsa-miR-663	3.12	10.69	-0.13	hsa-miR-339-5p	4.03	15.63	0.89
hsa-miR-1281	2.93	14.72	-0.74	hsa-miR-140-3 p	3.56	13.30	0.41
hsa-miR-422a	2.56	14.41	-0.69	hsa-miR-197	3.36	14.08	0.63
hsa-miR-1228-star	2.41	16.62	-0.98	hsa-miR-362-5p	3.01	15.25	0.86
hsa-miR-1975	2.10	12.84	-0.38	hsa-miR-324-5p	2.75	17.85	1.06
hsa-miR-1826	2.09	14.18	-0.67	hsa-miR-34a	2.70	23.46	2.32
hsa-miR-1915	2.08	10.83	-0.14	hsa-miR-210	2.17	13.30	0.44
hsa-miR-4284	2.07	14.34	-0.65	hsa-miR-500-star	2.06	11.06	0.18
hsa-miR-203	1.57	26.13	-3.84	hsa-miR-130a	1.77	22.49	2.13
				hsa-miR-130b	1.61	17.34	0.85
				hsa-miR-18a	1.60	13.41	0.46
				hsa-miR-29a	1.57	15.46	0.80
				hsa-miR-149	1.54	11.96	0.32

Differentially expressed microRNAs (miRNAs) in SP and MP cells of the gastric cancer cell line MKN-45 as determined by miRNA microarray and quantitative real-time-PCR analyses. These results showed that the miRNA profiles of SP cells differed from the miRNA profiles of MP cells. Moreover, downregulated miRNAs, such as hsa-miR-3175 and hsa-miR-203, and upregulated miRNAs, including hsa-miR-130a, hsa-miR-324-5p, hsa-miR-34a, and hsa-miR-25-star, showed significant differences in expression between SP and MP cells when tested by quantitative real-time PCR. SP: Side population; MP: Major population; PCR: Polymerase chain reaction.