Degradation of intestinal mRNA: A matter of treatment

Table 1 Real time PCR from muscle and submucosal/mucosal layer of individual gut layers

Tissue	Gene	Cp (RIN = 9)	Cp (RIN = 2)
Muscle	β-Tubulin III	22.07 ± 0.25	24.41 ± 0.22
Submucosal/mucosal	β-Tubulin III	27.42 ± 0.19	26.00 ± 0.93
Muscle	PGP 9.5	20.28 ± 0.09	23.67 ± 0.58
Submucosal/mucosal	PGP 9.5	25.43 ± 0.54	25.28 ± 0.58
Muscle	S100b	26.65 ± 0.47	29.07 ± 0.02
Submucosal/mucosal	S100b	28.53 ± 0.63	29.75 ± 1.30
Muscle	GAPDH	18.85 ± 0.05	18.68 ± 0.08
Submucosal/mucosal	GAPDH	18.93 ± 0.04	19.53 ± 0.20

The expression patterns in terms of cycles do depend on the RNA integrity number (RIN). Cp (RIN = 9): Quantification cycle by qPCR from RNA with a RIN of 9; Cp (RIN = 2): Quantification cycle by qPCR from RNA with a RIN of 2.