Anti-proliferation effects of Twist gene silencing in gastric cancer SGC7901 cells

Figure 3 Cell cycle arrest and apoptosis of Twist RNAi SGC-7901 cells. A: Representative histograms of cell cycle distribution, which show the percentages of cell-gated populations in the G0/G1, S, and G2/M phases by flow cytometric analysis; B: Bar graph of cell distributions with mean values and SD calculated from triplicate independent experiments; C: Representative DAPI staining results which show homogeneous staining of nuclei in control cells and irregular staining with small highlighted bodies as a result of apoptosis in Twist RNAi cells; D: Representative photomicrographs of positive staining (dark brown) in apoptotic cell nuclei by terminal deoxynucleotidyl transferase-mediated digoxigenin-dUTP nick-end labeling (TUNEL) assay.