Oleanolic acid and ursolic acid inhibit proliferation in transformed rat hepatic oval cells

Figure 4 Growth inhibitory effects of oleanolic acid and ursolic acid on H₂O₂-treated WB-F344 cells. A: Flow cytometric analyses of the cell cycle of H₂O₂-treated WB-F344 cells following treatment with oleanolic acid (OA) (4 μmol/L), ursolic acid (UA) (8 μmol/L) or 5-FU (0.05 mmol/L, positive control). After 72 h, the cells were harvested and stained with 10 μg/mL propidium iodide (PI), and the DNA content was analyzed, as described in the Materials and Methods; B: Cell cycle quantitative analysis. Histograms indicating the fraction of H₂O₂-treated WB-F344 cells and those that were treated with OA, UA or 5-FU in the G₁, G₂/M and S phases. The data represent the means ± SD derived from three independent experiments. ^aP < 0.05 vs H₂O₂ model group.