N-acetylcysteine attenuates reactive-oxygen-species-mediated endoplasmic reticulum stress during liver ischemia-reperfusion injury

Figure 3 Reactive oxygen species-mediated endoplasmic reticulum stress is inhibited by N-acetylcysteine treatment in liver after ischemia-reperfusion. A: Endoplasmic reticulum stress related gene (GRP78, ATF4 and CHOP) expressions in livers harvested 6 h after reperfusion, by quantitative real-time reverse transcriptase polymerase chain reaction analysis; mean ± SD, n = 3-5/group, ^bP < 0.01 vs sham group; ^dP < 0.01 vs IR group; B: western blot-assisted analysis of GRP78, ATF4, CHOP and β-actin; C: Relative quantities of protein of GRP78, ATF4 and CHOP, mean ± SD, n= 3-5/group, ^bP < 0.01 vs sham group; ^dP < 0.01, ^cP < 0.05 vs IR group; representative of three experiments. NAC: N-acetylcysteine; IR: Ischemia-reperfusion.