Role of ERK-MAPK signaling pathway in pentagastrin-regulated growth of large intestinal carcinoma

Table 1 Primers used for nested reverse transcriptase polymerase chain reaction amplification of cholecystokinin-B receptor, extracellular signal-regulated protein kinase 1/2 and K-ras

Name	Primer sequence	PCR conditions	Size (bp)
CCK-BR	1: 5’TCTCGCGAGCTCTACTTAGGG3’	94 °C, 30 s	185
	2: 5’ACCGACGATGCACGTTGAAG3’	62 °C, 30 s
		72 °C, 30 s
ERK1/2	1: 5’TATTCCCGGGCAAGCACTATTT3’	94 °C, 30 s	243
	2: 5’CGGGCTCATCATTCGGGTCGTA3’	54 °C, 30 s
		72 °C, 30 s
K-ras	1: 5’ACAGTGCAATGAGGGACCAGTA3’	94 °C, 30 s	275
	2: 5’GTATAGAAGGCATCATCAACACC3’	50 °C, 30 s
		72 °C, 30 s
Actin	1: 5’ATGATATCGCCGCGCTCGTCGTC3’	94 °C, 30 s	342
	2: 5’CGCGGTTGGCCTTGGGGTTCAG3’	60 °C, 30 s
		72 °C, 30 s

PCR: Polymerase chain reaction; CCK-BR: Cholecystokinin-B receptor; ERK1/2: Extracellular signal-regulated protein kinase 1/2.