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Copyright ©2014 Baishideng Publishing Group Inc.
World J Gastroenterol. Sep 14, 2014; 20(34): 12007-12017
Published online Sep 14, 2014. doi: 10.3748/wjg.v20.i34.12007
Table 3 Circulating microRNAs as diagnostic biomarkers
Ref.SampleMethod (normalization)MiRNAClinical application
Li et al[74] (CN)80 EGC/70 C plasmareal-time qRT-PCR(U6, U44)miR-199-3pEarly detection of GC
Rotkrua et al[72] (JP)6 DCKO mice with DGC/6 C tissuemiRNA microarray andmiR-103, miR-107,Early detection of DGC
21 DCKO mice with DGC/23 C serumTaqMan qRT-PCR (cel-miR-39)miR-194 and miR-210
Li et al[75] (CN)230 GC/130 C andmiRNA microarray andmiR-199-3pGC detection
20 gastric precancerous plasmareal-time qRT-PCR (U6, U44)
Cai et al[76] (CN)90 GC(70A, 16M, 3S, 1SC)/90 C plasmaqRT-PCR (cel-miR-39)miR-106b, miR-20a,Early detection of GC
and miR-221
Li et al[91] (CN)8 GC/8 C tissueqRT-PCR (U6, cel-miR-39)miR-223, miR-21GC detection
70 GC(56A, 13M, 1S)/70 C plasmaand miR-218
Wang et al[92] (CN)174 solid cancers/39 C serumqRT-PCR (miR-16)miR-21Detection of some solid cancers
Song et al[93] (CN)82 GC(71A, 3S, 1SC)/46 DYS/128 C serumTaqMan low-density arraymiR-221, miR-376cEarly detection of GC
and TaqMan qRT-PCR (cel-miR-39)and miR-744
Konishi et al[94] (JP)56 GC/30 C plasmamiRNA microarraymiR-451 and miR-486Screening GC
and qRT-PCR (U6)
Liu et al[77] (CN)61 GC(A, M)/61 C serummiRNA microarraymiR-378Early detection of GC
and real-time qRT-PCR (U6)
Liu et al[82] (CN)164 GC/127 C serumSolexa sequencingmiR-1, miR-20a, miR-27a,GC detection
and qRT-PCR (the serum volume)miR-34 and miR-423-5p
Tsujiura et al[73] (JP)8 GC/8 C tissueqRT-PCR (RNU6B)miR-17-5p, miR-21, miR-106a,GC detection
79 GC/30 C plasmamiR-106b and let-7a
Gorur et al[95] (TR)20 GC/190 C plasmareal-time qPCR (global means)miR-195-5pEarly detection of GC
Wang et al[54] (CN)80 gastric adenocarcinomas/40 peritumoral tissuesreal-time qRT-PCR (U6)let-7aGC detection
80 gastric adenocarcinomas/45 C serum