MicroRNAs as potential biomarkers for gastric cancer

Table 2 Tissue microRNAs as prognostic biomarkers

Ref.	Sample	Method (normalization)	MiRNA	Clinical application
Wang et al[42] (CN)	110 GC/110 C	qRT-PCR (U6)	miR-17-5p/20a	Therapeutic marker
Yan et al[66] (CN)	31 with/43 without recurrent GC	miRNA microarray and qPCR (U6)	miR-335	Recognition of recurrence risk
Liu et al[62] (CN)	92 GC/92 C	real-time qRT-PCR (U6)	miR-221	Prognostic factor for overall survival
Inoue et al[96] (JP)	161 GC/161 C	miRNA qPCR array, Taqman miRNA assay and real-time qRT-PCR (U6)	miR-107	Prediction of prognosis
Brenner et al[97] (IL)	45 GC (39A, 6S)	miRNA microarray and qRT-PCR (six miRs)	miR-451, miR-199a-3p and miR-195	Prediction of recurrence
Zhang et al[68] (CN)	29 with/36 without recurrent GC (A)	miRNA microarray and qPCR (U6)	miR-375 and miR-142-5p	Prediction of recurrence risk for GC
Kim et al[99] (KR)	15 GC cell lines	miRNA methylome profiling, bisulfite pyrosequencing and qRT-PCR (U6)	miR-10b	Assessing the risk of GC
	100 GC(44I, 56D)/100 C tissues
Nishida et al[49] (JP)	87 GC	qRT-PCR (RNU6B)	miR-125a-5p	Prognostic marker
Li et al[65] (CN)	100 GC	real-time RT-PCR	miR-10b, miR-21, miR-223, miR-338, let-7a, miR-30a-5p, miR-126	Survival prediction
Xu et al[64] (CN)	86 GC	RT-PCR (Let-7a)	miR-21	Prediction of LN metastasis
Wu et al[63] (CN)	52 GC/52 C	real-time RT-PCR (U6, Let7a, miR-191, 103)	miR-212 and miR-195	Prediction of LN metastasis
Liu et al[102] (CN)	84 GC/84 C	qRT-PCR (U6)	let-7i	Prediction of chemotherapeutic sensitivity and prognosis
Chen et al[61] (CN)	3 GC/1 gastric mucosal lines	miRNA array and qPCR (U6)	miR-10a	Prediction of LN metastasis
	38 GC (28A, 8T, 2S)/10 C tissues
Valladares-Ayerbes et al[100] (ES)	28 CC/7 GC/3 pancreas	qRT-PCR (5S and U6)	miR-17-92	Prognostic markers
Kogo et al[48] (JP)	90 GC/90 C	qRT-PCR (RNU6B)	miR-146a	Prognostic factor
Sacconi et al[46] (IT)	123 GC (75I, 24D, 24U)/123 C	miRNA microarray and qPCR (RNU6B)	miR-204	Prognostic value

C: Control; CC: Colorectal cancer; GC: Gastric cancer; LN: Lymph node; A: Adenocarcinoma; T: Tubular adenocarcinoma; S: Signet-ring cell carcinoma; I: Intestinal; D: Diffuse; U: Undefined.