Perinodular ductular reaction/epithelial cell adhesion molecule loss in small hepatic nodules

Figure 1 Immunohistochemistry staining pictures. Diffuse loss of DR/EpCAM in HCC > 3 cm (A, IHC, × 400), a surgical sample of HCC ≤ 3 cm (a moderately differentiated HCC), and the area of foci invasion (black arrow) in HCC ≤ 3 cm; the invasive area showed diffuse loss of DR/EpCAM; but positive on the biliary cells between hepatic lobules (white arrow) (B, IHC, × 400). Active DR/EpCAM was observed in a cirrhotic nodule (C, IHC, × 200), and part of liver cells were EpCAM positive (circle). DR/EpCAM was focally positive (D, IHC, × 400), and the cells showed features of hepatocyte inside the nodules of HCCs. HCC: Hepatocellular carcinoma; DR: Ductular reaction; EpCAM: Epithelial cell adhesion molecule; IHC: Immunohistochemistry.