Dihydromyricetin inhibits migration and invasion of hepatoma cells through regulation of MMP-9 expression

Figure 3 Dihydromyricetin inhibits cell migration in vitro. The Boyden transwell system was used to assay cell migration as described in the ‘‘MATERIALS AND METHODS’’. SK-Hep-1 (A) and MHCC97L (C) cells that passed through polycarbonate membranes with an 8.0-μmol/L pore size were fixed in 75% ethanol and stained with hematoxylin and eosin solution. The number of SK-Hep-1 (B) and MHCC97L (D) cells that migrated through the membrane was quantified by determining the number of cells per field under an inverted microscope at 100 × magnification. Dihydromyricetin (DHM) significantly suppressed the migration of SK-Hep-1 and MHCC97L cells. Each experiment was carried out at least three times. ^bP < 0.01 vs control, Student’s t test.