Effect of a high-fat diet in development of colonic adenoma in an animal model

Figure 5 Expression of proliferating cell nuclear antigen and cyclooxygenase -2 in colonic adenoma and normal control tissue for both normal-fat diet and high-fat diet groups by immunofluorescence staining. A: Representative images of immunofluorescence staining for proliferating cell nuclear antigen (PCNA); B: Representative images of immunofluorescence staining for cyclooxygenase -2 (COX-2). The proteins are stained red with corresponding secondary antibody conjugated with Cy3, and cell nuclei are stained blue with 4,6-diamidino-2-phenylindole (DAPI). Imaging was documented at × 400 magnification. ND: Normal-fat diet; HFD: High-fat diet.