Differential gene expression of chemokines in KRAS and BRAF mutated colorectal cell lines: Role of cytokines

Figure 7 Shows Caco2 (Wt) (A), DLD-1 (KRAS) (B) and HT-29 (BRAF) (C) Western blotting analysis. The cytokines interleukin-1β (1 ng/mL), tumor necrosis factor α (50 ng/mL) and interferon-γ (50 ng/mL) were stimulated to the cells and the total cell lysates was isolated and 20 μg were separated by 15%-20% NuPAGE Bis-Tris gel electrophoresis, blotted and probed with chemokine (C-X-C motif) ligand (CXCL) 10 antibody. β-actin (43 kDa) was analyzed as an internal control.