ALK gene copy number gain and its clinical significance in hepatocellular carcinoma

Figure 1 Representative fluorescent in situ hybridization images of tumors negative for anaplastic lymphoma kinase translocation. The two tumors varied in copy number, but both were negative for anaplastic lymphoma kinase (ALK) translocation, as indicated by the signals that neither had green and orange in close proximity or were fused to form a yellow locus. A: ALK/copy number gain (CNG)-positive cells (≥ 4 copies/cell); B: ALK/CNG-negative cells (< 4 copies/cell); C and D: HE staining of hepatocellular carcinoma cells from A and B (× 200).