Clinicopathological and biological significance of cripto overexpression in human colon cancer

Figure 2 Effects of S1 siRNA on cripto mRNA of colon cancer LS-174T cells. The LS-174T colon cancer cell line was transfected with different dose of S1 siRNA for 24, 48, 72 h. Cripto mRNA was evaluated by real-time reverse transcription-polymerase chain reaction as described in “Materials and Methods,” using GAPDH as internal standard. All data are presented as the mean ± SD of three independent experiments; the level of cripto mRNA relative to GAPDH in untreated cells maintained under identical experimental conditions was taken as 100%.