Cdx2 expression and its promoter methylation during metaplasia-dysplasia-carcinoma sequence in Barrett's esophagus

Figure 2 Histological findings of transitional area between intestinal metaplasia and high-grade dysplasia or intramucosal adenocarcinoma (× 200). A: Hematoxylin and eosin staining of transitional area. Intestinal metaplasia (IM) stretches from the upper left to the lower right corner; B: Mucin (MUC) 5AC immunostaining. Strong MUC5AC expression is observed in both IM and high-grade dysplasia (HD), or intramucosal adenocarcinoma (IMC) areas; C: MUC6 immunostaining. MUC6 expression is observed mostly in parts of the HD or IMC areas; D: Caudal type homebox transcription factor 2 (Cdx2) immunostaining. Cdx2 expression is observed only in the nuclei of the cells in the IM area; E: E-cad immunostaining. E-cad expression is observed on the membranes of cells in both IM and HD or IMC areas; F: p53 immunostaining. Strong p53 expression is observed in the nuclei of the cells in the HD or IMC area.