Isolation and biochemical analysis of vesicles from taurohyodeoxycholic acid-infused isolated perfused rat livers

Table 1 High-performance liquid chromatography analysis of the fatty acid composition of phosphatidylcholine

Fractions	PC (nmol)	C16:0 C20:4	C16:0 C18:2	C16:0 C18:1
Control perfused fed rat livers
1	0.171 ± 0.001	41.147% ± 3.02%	11.048% ± 0.8%	8.327% ± 0.92%
2	0.223 ± 0.003	42.219% ± 2.8%	10.223% ± 0.7%	6.49% ± 0.5%
8	0.214 ± 0.007	41.545% ± 3.09%	11.281% ± 0.8%	6.436% ± 0.9%
9	1.02 ± 0.05	43.59% ± 1.5%	8.68% ± 1%	6.61% ± 0.73%
10	1.34 ± 1.1	44.64% ± 0.27%	8.19% ± 0.64%	6.84% ± 0.33%
THDC-perfused rat livers
1	0.193 ± 0.001	44.424% ± 3.29%	7.864% ± 0.92%	10.239% ± 1.52%
2	0.208 ± 0.004	44.796% ± 2.09%	8.177% ± 0.8%	8.507% ± 0.93%
8	0.444 ± 0.008	43.735% ± 0.98%	9.373% ± 1.76%	8.298% ± 1.35%
9	1.47 ± 0.16	44.94% ± 1.2%	8.07% ± 0.3%	8.23% ± 0.95%
10	1.17 ± 0.4	43.94% ± 2.8%	7.94% ± 0.52%	7.74% ± 1.7%

Phosphatidylcholine was isolated and separated by thin layer chromatography and then subjected to high-performance liquid chromatography (HPLC) for the sub-fractionation of the fatty acid composition as described in the methods section for control perfused livers and in livers perfused with taurohyodeoxycholic acid (THDC). Results are presented as mean ± SE (n = 6). No significant differences were found between the fatty acid composition of livers perfused with THDC when compared to the control; PC: Phosphatidylcholine.