miRNA-338-3p suppresses cell growth of human colorectal carcinoma by targeting smoothened

Figure 4 Effects of miRNA-338-3p on cell proliferation and apoptosis in colorectal carcinoma cells. A: Cell proliferation was determined by 3-(4,5-dimethyl-2 thiazoyl)-2,5-diphenyl-2H-tetrazolium bromide assay. Cellular proliferation inhibition rate (CPIR) in the presence of pre-miRNA-338-3p (miR-338-3p) or miR-338-3p-inhibitor was compared with that of the controls; n = 6, mean ± SD. ^bP < 0.01 vs control group; B: Effects of pre-miR-338-3p and miR-338-3p-inhibitor on cell-cycle in SW-620 cells. The percentages of cells in G₀/G₁ phase and S phase and apoptotic rate were measured by computing the ratio of the number of corresponding cells to total cells; n = 3, mean ± SD. ^bP < 0.01 vs control group; C-F: Apoptosis analysis of transduced cells by flow cytometry. C: Blank control; D: SW-620 cells transduced with lentivirus pLV-THM-control; E: SW-620 cells transduced with lentivirus pLV-THM-miR-338-3p; F: SW-620 cells transduced with lentivirus pLV-THM-miR-338-3p-inhibitor. The right lower quadrant (FITC⁺/PI^-) shown as apoptotic cells.