S100A4 silencing blocks invasive ability of esophageal squamous cell carcinoma cells

Figure 1 S100A4 and E-cadherin expression in metastasis and non-metastasis tissue. A: The representive reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting results for S100A4 and E-cadherin in metastasis tissue; B: The representive RT-PCR and Western blotting results for S100A4 and E-cadherin in non-metastasis tissue; C and D: Levels of transcripts of S100A4 and E-cadherin in M tumor samples in comparison to N tumor (expressed as transcript copynumber per 50 μg of messenger RNA and standardized with β-actin). The intensity of each band relative to the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (α-tubulin) band was represented as the mean ± SD, ^aP < 0.05 vs metastasis group and ^bP < 0.05 vs non-metastasis group.