Effects of resistin-like molecule β over-expression on gastric cancer cells in vitro

Figure 3 Over-expression of resistin-like molecule β attenuated the adhesion, migration and invasion of gastric cancer cells in vitro. A: In the adhesion assay, SGC-7901 and MKN-45 cells transfected with pcDNA3.1-resistin-like molecule β (RELMβ) for 72 h exhibited markedly reduced ability in adhesion to the precoated matrigel, when compared with parental cells. However, the cells transfected with empty vector (mock) had a similar adhesive ability as parental cells; B: Scratch migration assay indicated that transfection of pcDNA3.1-RELMβ into SGC-7901 and MKN-45 cells for 72 h resulted in an impaired migration capacity, when compared with the parental cells and mock group; C: Transwell analysis indicated that transfection of pcDNA3.1-RELMβ for 72 h abolished the invasive capabilities of SGC-7901 and MKN-45 cells, when compared with the parental and mock cells. The symbol (a) indicates a significant decrease compared with parental cells (P < 0.01). Triplicate experiments were performed with essentially identical results.