Glycyrrhizin attenuates HMGB1-induced hepatocyte apoptosis by inhibiting the p38-dependent mitochondrial pathway

Figure 2 High-mobility group box 1-induced hepatocyte apoptosis is p38-dependent. A: Huh-BAT cells were treated with 10 μg/mL of high-mobility group box 1 (HMGB1) for the indicated time periods. Cells were lysed at the indicated time points, and immunoblot analysis was performed on cell lysates using antibodies specific for the phosphorylated forms of p42/p44, p38, or c-Jun N-terminal kinase (JNK); B: Huh-BAT cells were pretreated with mitogen activated protein kinase inhibitors U0126 (30 μmol/L), SB203580 (10 μmol/L), or SP600125 (20 μmol/L), or media (control) for 12 h. Cells were then treated with 10 μg/mL of HMGB1. Cells were lysed at the indicated time points, and immunoblot analysis was performed on cell lysates using anti-caspase 3 and anti-actin antibodies.