Adeno-associated virus mediated delivery of Tregitope 167 ameliorates experimental colitis

Figure 1 Schematic overview of the trinitrobenzene sulfonate induced colitis model. Mice were injected intravenously with either phosphate-buffered saline (PBS) or adeno associated virus (AAV) 5 [cytomegalovirus (CMV) promoter T-cell epitopes (Tregitope) 167]. Ten days after AAV-mediated Tregitope delivery acute colitis was initiated by intra-rectal administration of 1.5 mg trinitrobenzene sulfonate (TNBS) in 40% ethanol followed by a second TNBS treatment (0.75 mg in 20% ethanol) 8 d later. Control groups consisted of mice not treated by TNBS (healthy control group) and mice treated by TNBS only (diseased control group).