siRNA-mediated downregulation of TC21 sensitizes esophageal cancer cells to cisplatin

Figure 5 Cell cycle analyses. A: TC21-knockdown cells (20 nmol siRNA for 48 h) were treated with cisplatin (8 μg/mL) for 24 h and cell cycle analysis was performed by labeling cells with DNA binding dye propidium iodide. The panel shows 1: DNA histograms of mock-transfected TE13 cells for 48 h; 2: TE13 cells transfected with TC21 siRNA for 48 h showing increased cell death; 3: TE13 cells without transfection for 24 h; 4: TE13 cells were treated with cisplatin alone for 24 h; 5: TE13 cells without transfection for 72 h; 6: TE13 cells were transfected with TC21siRNA for 48 h for optimal knockdown followed by cisplatin at 8 μg/mL for 24 h; B: Stacked bar graph depicts the cell cycle distribution. Data presented here as mean of percentage events from 3 independent experiments.