Mutual regulation between microRNA-373 and methyl-CpG-binding domain protein 2 in hilar cholangiocarcinoma

Figure 5 Enrichment of methyl-CpG-binding domain protein 2 at CpG island region is essential for methylation-mediated silencing of miR-373 gene. A: Enrichment of methyl-CpG-binding domain protein (MBD)2 in fragment of CpG island in HEK-m373-prom stable cells showed 6.2-fold increase compared to control and no significant change was detected in HEK-u373-prom cells; B: MBD2 enrichment in MBD2-siRNA QBC₉₃₉ cells presented dramatically deduction compared to QBC₉₃₉ cells; C: Knock-down of MBD2 induced a increase of miR-373 expression in QBC₉₃₉ cells; D: Epigenetic treatment of QBC₉₃₉ cell with 5-Aza-CdR or combination with trichostatin A (TSA) eliminated the recruitment MBD2 at the region of miR-373 promoter-associated CpG island. ^bP < 0.01 vs background.