Effect of biologically active fraction of Nardostachys jatamansi on cerulein-induced acute pancreatitis

Figure 8 Effects of the 4th fraction of Nardostachys jatamansi on heme oxygenase-1 expression in acinar cells and cerulein-induced acinar cell death. Pancreatic acinar cells were pretreated with Nardostachys jatamansi (NJ) (20 μg/mL), and then the cells were harvested at the indicated time. A, B: The protein level (A) and mRNA level (B) of heme oxygenase-1 (HO-1) in pancreatic acini were measured. Pancreatic acinar cells were pretreated with the indicated dose of NJ4. Then, the cells were harvested at 6 h; C, D: The protein level (C) and mRNA level (D) of HO-1 in the pancreatic acini were detected. The pancreatic acinar cells were pretreated with the indicated dose of NJ4 and then stimulated with cerulein (10 nmol/L); E: After 6 h of cerulein stimulation, cell viability was measured as described in the experimental protocol. Pancreatic acinar cells were pretreated with ZnPP (10 μmol/L), an HO-1 inhibitor, for 1 h and then treated with NJ4 (20 μg/mL), curcumin (10 μmol/L); F: At 1 h after treatment, cerulein (10 nmol/L) was added; After 6 h of cerulein stimulation, cell viability was measured. The results were similar in 3 additional experiments. ^aP < 0.05 vs the saline treatment; ^cP < 0.05 vs cerulein treatment alone. Cur: Curcumin.