Germline promoter hypermethylation of tumor suppressor genes in gastric cancer

Figure 3 Germline CDH1 promoter hypermethylation analysis in GC patients. A: Map of the CDH1 promoter region and primer positions. The sequence is numbered relative to the transcription start site for human CDH1. Characters in red indicate the primer binding sites for methylation-specific PCR (MSP), those in blue for bisulfite sequencing. Individual CpG sites in the sequence are numbered consecutively; B: MSP of the CDH1 promoter from the peripheral blood of GC patients. Marker, DL2000 DNA Markers (TaKaRa); +, CpGenome Universal Methylated DNA control; -, CpGenome Universal Unmethylated DNA control; M, methylated band; U, unmethylated band. As a control, the fragments corresponding to the CDH1 promoter of fully methylated DNA showed a clear band when amplified with methylated-specific primers and did not display a band when treated with unmethylated primers. The unmethylated DNA control showed the reverse pattern.