Glutamine depletion induces murine neonatal melena with increased apoptosis of the intestinal epithelium

Figure 3 Apoptotic changes observed in the damaged colonic epithelium. Electron micrographs of colonic epithelia obtained from infant mice with glutamine-rich milk (GRM) (A, D), complete amino acid milk (CAM) (B, E), and glutamine-deleted milk (GDM) (C, F) at low magnification (A-C) and high magnification (D-F). Asterisks (*) represent lipid droplets and the arrow shows an apoptotic cell. G-I: Optical microscopic views (magnification, × 200) of immunohistochemistry for cleaved caspase-3 as a marker of apoptosis using resected tissues from the same mice (G: GRM, H: CAM, I: GDM).