Octreotide induces caspase activation and apoptosis in human hepatoma HepG2 cells

Figure 4 The apoptotic effect of octreotide and tumor necrosis factor-α alone is shown. A: Octreotide and tumor necrosis factor-α (TNF-α) significantly increased early (Annexin-V only positive, right lower quadrant) and more so late apoptotic cells [Annexin-V and propidium iodide (PI) positive, right upper quadrant]. Every sample was analyzed with the same gating strategy (Gate A) to exclude debris and non-specific binding of Annexin-V, while control refers to uninduced HepG2 cells; B: Annexin-V positive cells; C: Annexin-V/PI positive cells. B and C: Mean of 8 different experiments ± SE (^bP < 0.01, ^dP < 0.001).