Helicobacter pylori arginase mutant colonizes arginase II knockout mice

Figure 5 Ex vivo arginase activity of organs from arginase II knockout mice fed water or the arginase inhibitor S-(2-boronoethyl)-L-cysteine. Mice were administered 20 μL water or 50 mmol/L S-(2-boronoethyl)-L-cysteine (BEC) once or twice daily for 3 d by direct oral delivery via pipetting. Animals were euthanized and organs removed and homogenized. The data represent the average arginase activity of four to five mice ± SD, with each mouse organ measured in duplicate or triplicate using arginine buffer at pH 9.0 in the presence of manganese. A: Liver. Arginase activity was inhibited almost 50% in the liver by BEC. P = 0.0006 between the two groups; B: Kidney. Arginase activity was inhibited > 75% in the kidney by BEC. P = 0.0033 between the two groups; C: Antrum. Arginase activity was inhibited > 75% in the antrum by BEC. P = 0.0007 between the two groups; D: Body. No inhibition was observed in the body (P > 0.05).