Legalon-SIL downregulates HCV core and NS5A in human hepatocytes expressing full-length HCV

Figure 5 Effects of legalon-SIL on HMOX-1, Bach1, and Nrf2 protein levels in CON1 cells after 48 h. CON1 cells were grown to near confluence and the medium was changed to 5% FBS plus Dulbecco’s modified Eagle’s medium, and then treated with vehicle only (DMSO) or 50 or 200 μmol/L legalon-SIL (LS). The cells were harvested 48 h after treatment. Total protein (10 μg) was separated on 4%-12% gradient sodium dodecyl sulphate-polyacrylamide gel electrophoresis and electrophoretically transferred onto an Immun-Blot PVDF. HMOX-1, Bach1, and Nrf2 protein levels were assessed through Western blotting, and normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). A: Representative Western blottings. B: Results of these independent experiments quantified and normalized to GAPDH. Data for protein levels are mean ± SE (n = 3 independent experiments). ^aP < 0.05 vs DMSO control.