Epigallocatechin gallate inhibits HBV DNA synthesis in a viral replication - inducible cell line

Figure 5 Epigallocatechin gallate down-regulates hepatitis B virus covalently closed circular DNA and replicative intermediates of DNA level. A: Epigallocatechin gallate (EGCG) treatment was the same as described in Figure 1. Hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) and total cellular HBV DNA were extracted and subjected to real-time polymerase chain reaction analysis; B: Lamivudine, the potent HBV DNA synthesis inhibitor, was used as a control inhibitor in this analysis. DNA level in the no treatment control was arbitrarily designated as 1. Error bars indicate standard deviation of 3 independent experiments. RI-DNA: Replicative intermediates of DNA.