Catalytic domain of PDC-E2 contains epitopes recognized by antimitochondrial antibodies in primary biliary cirrhosis

Table 3 Reactivity and incidence of IgM-antibodies to 33 PDC-E2 peptides in PBC patients as compared to healthy individuals n (%)

	Peptide No.	Amino acid	PBC patients		Healthy individuals (n = 22)
			Anti-M2+ (n = 95)	AMA-/ANA+ (n = 11)
Outer lipoyl domain (aa 1-80)	1	-2-23	7 (7)	0	1 (5)
	2	16-40	10 (11)	0	2 (9)
	3	33-57	27 (28)	0	0
	4	50-74	33 (35)	1 (9)	1 (5)
	5	67-91	42 (44)	0	1 (5)
Hinge	6	84-108	31 (33)	0	1 (5)
	7	101-125	52 (55)1	1 (9)	2 (9)
Inner lipoyl domain (aa 133-217)	8	118-142	34 (36)	0	1 (5)
	9	135-159	35 (37)	0	2 (9)
	10	152-176	35 (37)	0	1 (5)
	11	169-193	37 (39)2	0	2 (9)
	12	186-210	27 (28)	0	1 (5)
	13	203-227	23 (24)	0	2 (9)
Hinge	14	220-244	26 (27)	0	0
	15	237-261	43 (45)	0	3 (14)
	16	254-278	33 (35)	0	1 (5)
E1/E3-binding site (aa 272-303)	17	271-295	15 (16)	0	0
	18	288-312	24 (25)	0	1 (5)
Hinge	19	305-329	36 (38)	0	3 (14)
Catalytic site (aa 331-561)	20	322-346	1 (1)	0	0
	21	339-363	4 (4)	0	0
	22	356-380	24 (25)	0	1 (5)
	23	373-397	10 (11)	0	0
	24	390-414	23 (24)	1 (9)	3 (14)
	25	407-431	55 (58)3	0	1 (5)
	26	424-448	21 (22)	0	0
	27	441-465	16 (17)	0	0
	28	458-482	15 (16)	0	0
	29	475-499	70 (74)4	0	1 (5)
	30	492-516	14 (15)	0	2 (9)
	31	509-533	6 (6)	0	2 (9)
	32	526-550	4 (4)	0	0
	33	543-561	9 (9)	0	1 (5)

^1,2,3,4Same as Table 2. Peptides 3, 7, 10, 11, 25 and 29 are previous and novel immunodominant epitopes.