Comparative analysis of dideoxy sequencing, the KRAS StripAssay and pyrosequencing for detection of KRAS mutation

Table 1 Sensitivity of the 3 methods in mutation detection

Methods	Mutant DNA/total DNA (%)
	100	50	20	15	10	5	1	0
Dideoxy sequencing	Yes	Yes	Yes	Yes	No	No	No	No
Pyrosequencing	Yes	Yes	Yes	Yes	Yes	Yes	No	No
KRAS StripAssay	Yes	Yes	Yes	Yes	Yes	Yes	Yes	No

Dideoxy sequencing could identify mutations in dilutions containing ≥ 15% mutant DNA, pyrosequencing could identify mutation in dilutions containing ≥ 5% mutant DNA, the KRAS StripAssay could identify mutant DNA as low as 1%.