Helicobacter pylori HopE and HopV porins present scarce expression among clinical isolates

Figure 3 Detection of hopE and hopV genes and their expression in H. pylori clinical isolates from different Chilean cities. Amplicons were separated in 1% agarose gels. A: PCR detection of the hopE gene; B: PCR detection of the hopV gene. Arrows indicate migration of the respective gene fragments. Lane Std: 1 kb DNA ladder standard (Fermentas); Lane Std2: Lambda DNA/HindIII marker (Fermentas); C: Expression of HopV and HopE porins in H. pylori Chilean strains separated by 12% SDS-PAGE gels and detected by Western blots with respective polyclonal antibodies. Clinical isolates are indicated under the respective lanes. Std: Prestained molecular weight marker (Fermentas). The CHCTX-1 strain was included as a positive control.