8-bromo-7-methoxychrysin-induced apoptosis of hepatocellular carcinoma cells involves ROS and JNK

Figure 6 Effect of N-acetylcysteine, an antioxidant, and GW9662, a blocker of peroxisome proliferator-activated receptor-γ, and Jun N-terminal kinase inhibitor SP600125 on 8-bromo-7-methoxychrysin-induced activation of Jun N-terminal kinase (A), apoptosis (B), activation of caspase-3 (C), and DNA fragmentation (D) in HepG2 cells. The ratio of p-Jun N-terminal kinase (JNK)/JNK or p-c-Jun/c-Jun was normalized to 0 h or the untreated group. ^aP < 0.05 vs treatment with dimethyl sulfoxide (DMSO); ^cP < 0.05 vs treatment with 8-bromo-7-methoxychrysin (BrMC) alone; ^eP < 0.05 vs treatment with N-acetylcysteine (NAC) in combination with SP600125 and BrMC or GW9662 in combination with SP600125 and BrMC.