CD74 is a survival receptor on colon epithelial cells

Figure 4 MIF induces cyclin E expression. CEC were isolated from C57BL/6 (A) and NOD/SCID (B) mice that were i.p injected with MIF (400 ng) or PBS 3.5 h prior to isolation of CEC. A, B: Quantitative real time PCR was performed using primers for cyclin E and β-actin. β-actin levels were used to normalize samples for calculation of the relative expression levels of cyclin E. Results are expressed as a fold of change in cyclin E expression at stimulated cells compared to non stimulated cells, which was defined as 1. Results shown are summary of four separate experiments.