Effect of severe acute pancreatitis on pharmacokinetics of Da-Cheng-Qi Decoction components

Table 2 Stability of the 6 major DCQD compositions in rat plasma QC samples (%, n = 3)

	Spiked amount(ng/mL)	Bench-top bias		Long-term bias		Freeze-thaw bias		Extract bias		Autosampler bias
		2 h	4 h	7 d	14 d	1	2	8 h	24 h	6 h	12 h
Rhein	39.1	-1.32	-4.57	-1.15	-4.27	-1.15	-2.54	6.06	-4.54	-5.25	-5.33
	156	-2.50	-5.39	-2.36	-0.43	-2.36	0.43	2.28	2.48	-1.29	-1.07
	625	5.28	-1.21	1.60	-2.82	1.60	-2.93	-0.90	3.40	-0.48	-0.27
	3750	2.06	5.52	-4.46	0.09	-4.46	-0.17	1.50	1.32	-1.54	-3.60
Chrysophanol	6.1	-0.67	1.00	2.42	3.54	2.42	0.90	4.73	3.19	0.73	2.36
	24.4	5.04	4.62	-2.74	-2.61	-2.74	-6.40	-2.82	-4.42	-3.39	-3.52
	97.7	-0.32	5.80	-5.39	0.27	-5.39	-1.52	-2.34	0.00	-3.41	0.00
	568.0	-0.72	-2.00	2.01	-2.13	2.01	-2.47	-2.92	-0.97	0.63	2.42
Rheochrysidin	6.1	1.59	-2.59	-4.11	-3.78	-4.11	-5.18	-3.93	-6.27	-6.33	-5.10
	24.4	4.09	-0.65	0.00	-4.03	0.00	-1.27	-0.43	3.24	-0.42	-3.18
	97.7	-0.86	-4.45	-0.38	0.59	-0.38	-2.43	-0.58	-0.32	0.38	-2.49
	568.0	2.80	-5.52	-3.40	-2.09	-3.40	-2.18	2.04	1.16	-1.66	-3.66
Magnolol	6.1	-1.58	1.33	-5.23	-4.34	-5.23	-3.09	-2.72	-2.14	-5.18	-4.60
	24.4	5.76	0.84	0.42	-2.49	0.42	0.28	-1.95	1.53	1.25	-0.83
	97.7	1.82	1.17	1.48	-1.10	1.48	0.90	4.23	1.47	-3.21	1.35
	568.0	-2.02	-3.03	-1.60	-4.43	-1.60	-2.21	0.23	-5.01	-3.82	-6.14
Hesperidin	6.1	-4.29	4.85	-0.48	-1.27	-0.48	-3.08	1.01	2.34	-3.98	-1.54
	24.4	-2.03	-3.11	0.41	0.00	0.41	-1.36	1.46	1.46	-4.21	4.76
	97.7	3.42	-1.69	0.11	0.39	0.11	1.37	2.67	2.33	2.70	5.12
	568.0	-3.00	-0.81	-4.73	-2.25	-4.73	-3.55	3.75	0.62	-2.76	0.23
Naringin	6.1	-1.65	2.70	-1.96	1.63	-1.96	-4.90	-1.73	-0.49	2.61	-2.83
	24.4	3.95	4.90	-4.56	-2.73	-4.56	-2.73	1.04	2.86	-4.69	-6.12
	97.7	0.07	2.03	-5.11	-0.96	-5.11	1.58	-4.46	-2.31	-0.45	0.93
	568.0	-4.10	1.68	-4.52	-1.45	-4.52	-4.81	1.20	-3.06	-2.08	-1.56