Macrophage migration inhibitory factor regulates proliferation of gastric cancer cells via the PI3K/Akt pathway

Figure 1 Effects of recombinant human migration inhibitory factor (rhMIF) and LY294002 on the proliferation of MGC-803 cells. A: MGC-803 cells were treated with 25, 50 and 100 μg/L rhMIF for the indicated periods of time and cell proliferation was examined by MTT assay. ^aP < 0.05 vs control group; ^cP < 0.05 vs 50 μg/L MIF group; ^eP < 0.05 vs 24 h group; B: With or without 1-h pretreatment with 25 μmol/L LY294002, MGC-803 cells were exposed to 50 μg/L rhMIF for the indicated periods of time, with a vehicle control of dimethyl sulfoxide (DMSO) (less than 0.1%) included. ^aP < 0.05 vs control group; ^cP < 0.05 vs MIF group; ^eP < 0.05 vs LY+MIF group. Data were represented as mean ± SD of three independent experiments. DMSO (the menstruum of LY294002, less than 0.1%) had little impact on the rhMIF effect.