Expression of γ-synuclein in colorectal cancer tissues and its role on colorectal cancer cell line HCT116

Figure 3 Inhibition of cell proliferation and colony formation by γ-synuclein knockdown. A: The role of γ-synuclein in regulating HCT116 cell proliferation was determined by CCK8 assay. Values were the mean ± SD of absorbance at 490 nm for five independent experiments; ^aP < 0.05; B: The colony formation rates were analyzed by soft agar assay. Values were the mean ± SD for three independent experiments; ^aP < 0.05; C: The colonies were stained with 0.2% crystal violet and photographed. Control: Parental HCT116 cells; pGCsi-vector: HCT116/vector cells; siRNA2: HCT116/siRNA2 cells.