Application of Stool-PCR test for diagnosis of Helicobacter pylori infection in children

Table 1 Primers sequences and PCR conditions

Primers	Sequences	Product size (bp)	PCR conditions
16sRNA	5'GCTAAGAGATCAGCCTATGTCC3'	500	95°C 5 min (1 cycle); 94°C for 1 min, 55°C for 1 min
	5'TGGCAATCAGCGTCAGGTAATG3'		72°C for 2 min (39 cycles); 72°C for 7 min
VacA (s)	5'ATGGAAATACAACAAACACAC3'	s1: 259	95°C 4 min (1 cycle); 95°C for 1 min, 52°C for 1 min
	5'CTGCTTGAATGCGCCAAAC3'	s2: 286	72°C for 1 min (35 cycles); 72°C for 10 min
vacA (m)	5'CAATCTGTCCAATCAAGCGAG34	m1: 570	95°C 4 min (1 cycle); 95°C for 1 min, 52°C for 1 min
	5'GCGTCTAAATAATTCCAAGG3'	m2: 642	72°C for 1 min (35 cycles); 72°C for 10 min
cagA	5'AATACACCAACGCCTCCA3'	400	94°C for 4 min (1 cycle); 94°C for 1 min, 59°C for 1 min
	5'TTGTTGCCGCTTTTGCTCTC3'		72°C for 1 min (35 cycles); 72°C for 10 min