Effects of lysophosphatidic acid on human colon cancer cells and its mechanisms of action

Figure 5 LPA protected cells from cisplatin- & 5-FU-induced apoptosis. SW480 cells were serum-starved for 24 h and treated for 24 h with 10 μg/mL cisplatin (CDDP); 10 μg/L cisplatin + 20 μmol/L LPA; 8 μg/mL 5-FU; 8 μg/mL 5-FU + 20 μmol/L LPA; 10 μg/mL cisplatin + 20 μmol/L LPA + 50 μmol/L LY294002; 10 μg/mL cisplatin + 20 μmol/L LPA + 10 μmol/L PD98059, respectively. Cells were harvested, stained with Annexin V/PI, and analyzed by FACS analysis. The Annexin V-positive and PI-negative population was defined as apoptotic cells. Representative results from three separate experiments are presented.