Original Articles
Copyright ©2009 The WJG Press and Baishideng.
World J Gastroenterol. Sep 28, 2009; 15(36): 4547-4555
Published online Sep 28, 2009. doi: 10.3748/wjg.15.4547
Figure 3
Figure 3 LPA stimulated migration of SW480 cells. A: LPA stimulated migration of SW480 cells (× 200). SW480 cells (1 × 105/100 μL) were seeded into the inserts of transwell chambers after starvation for 8 h. Cells were incubated at 37°C for 4 h. Cells on the outside surface of inserts were fixed and stained. Typical images are presented; B: Migrated cells were quantified and relative migration rates (mean ± SE) are presented. bP < 0.001 vs 0 μmol/L LPA; C: Effect of inhibitors on LPA-induced cell migration. 50 μmol/L LPA in 600 μL medium was added to the lower chamber of transwell. Inhibitors including 10 μmol/L PD98059, 50 μmol/L LY294002 and 10 μmol/L Y-27632 were added to cells in the upper chamber. Cells on the outside surface of inserts were fixed, stained, and quantified. Data were analyzed using one-way ANOVA with post-hoc t-tests. bP < 0.001 vs 50 μmol/L LPA.