Molecular determinants of the profibrogenic effects of endothelin-1 in pancreatic stellate cells

Figure 4 ET-1 activates DNA binding of AP-1. Serum-starved PSC growing in 6-well plates were incubated with ET-1 (100 nmol/L) for the indicated periods of time. Nuclear extracts were subjected to EMSA analysis using a [³²P]-labelled oligonucleotide with an AP-1 binding motif. Supershift analysis was performed by incubating the binding reaction with a c-Fos-specific antibody. Shifted complex and supershifted band (lane 6, faint since antibody binding may diminish binding of c-Fos to the oligonucleotide) are pointed out by arrows. Results are representative of three independent experiments.