Lentivirus-mediated shRNA interference targeting STAT3 inhibits human pancreatic cancer cell invasion

Figure 3 Expression of STAT3 suppressed by LV-siSTAT3-2 in SW1990 cells. A: SW1990 cells were infected with LV-Con or LV-STAT3siRNA-2; The cells were infected (MOI = 40), GFP expression and the phase contrast images were taken after 72 h (original magnification × 200). B: mRNA level of STAT3 after SW1990 cells were treated with LV-STAT3siRNA-2 and LV-Con detected by real-time PCR. LV-STAT3siRNA-2 significantly inhibited expression of STAT3 mRNA in SW1990 cells, ^aP < 0.05, compared with SW1990 cells, ^cP < 0.05, compared with LV-Con group. C: Western blotting analysis showed that STAT3 protein was markedly inhabited by LV-STAT3siRNA-2 in SW1990 cells.