Genomic-wide analysis of lymphatic metastasis-associated genes in human hepatocellular carcinoma

Table 2 Up-regulated genes correlated with lymphatic metastasis

Gene name	Symbola	Accessionb	Fold changec	pN1:pN0d
Cell adhesion proteins
CD58 antigen, (lymphocyte function-assocoated antigens)	CD58	NM_001779	7.75	2.28
Integrin αM	ITGAM	NM_000632	5.04	2.06
Integrin β5	ITGB5	NM_002213	3.98	1.87
Opioid-binding protein/cell adhesion molecule-like	OPCML	NM_002545
Cell membrane receptor
CD86 antigen, (CD28 antigen ligand 2, B7-2 antigen)	CD86	NM_006889	6.76	2.33
v-jun sarcoma virus 17 oncogene homolog (avian)	JUN	NM_002228	7.43	2.27
Met proto-oncogene (hepatocyte growth factor receptor)	MET	NM_000245	10.11	3.46
EphA2	EPHA2	NM_004431	8.26	2.13
Epidermal growth factor receptor [avian erythroblastic leukemia viral (v-erb-b) oncogene homolog]	EGFR	NM_005228	8.35	2.62
Cell death regulator
BCL2/adenovirus E1B 19kDa interacing protein 3	BNIP3	NM_004052	3.88	2.29
Sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D	SEMA4D	NM_006378	5.71	3.64
Intracellular signaling
Rho GDP dissociation inhibitor γ	ARHGDIG	NM_001175	5.37	1.97
Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3)	RAC3	AK054993	4.63	2.07
Insulin-like growth factor binding protein 3	IGFBP3	M35878	5.39	2.15
Coagulation factor II (thrombin) receptor	F2R	NM_001992	6.17	2.43
Growth/differentiation factor
Vascular endothelial growth factor C	VEGFC	NM_005429	5.68	2.47
Cell cycle regulator
Cyclin D1 (PRAD1: parathyroid adenomatosis 1)	CCND1	NM_053056	11.56	4.31
Cyclin-dependent kinase 4	CDK4	NM_000075	8.73	3.59
Others
Tissue inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy, pseudoinflammatory)	TIMP3	NM_000362	3.95	1.89
Ubiquitin-conjugating enzyme E2A (RAD6 homolog)	UBE2A	NM_003336	4.38	1.95
v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1	YES1	NM_005433	5.66	2.37
P450(cytochrome) oxidoreductase	POR	AF258341	4.74	1.99
Matrix metalloproteinase 13	MMP13	NM_00247	5.67	2.45
Matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IV collagenase)	MMP2	NM_004530	7.13	3.36

^aSymbol in LocusLink database;

^bGeneBank accession number;

^cFold change, ratio of mean expression values in lymph node metastasis cases (cancer cells vs non-cancerous cells);

^dpN1:pN0, ratio of mean expression values (lymph node positive cases to lymph node negative cases).