Insulin-like growth factor binding protein-5 influences pancreatic cancer cell growth

Figure 8 Effect of IGFBP-5 on signaling in serum-starved PaC cells. BxPC-3 (A) and PANC-1 (B) stable transfectants [empty vector- (-), low IGFBP-5-L, or high IGFBP-5-H] were deprived of serum for 24 h then incubated in the presence of vehicle (DMSO), LY294002, or U0126 (10 &mgr;mol/L) for an additional 48 h. Cell lysates were prepared for immunoblot analysis of Akt or ERK1/2 activation using phospho-specific antibodies. Membranes were subsequently stripped and reprobed for total Akt or ERK1/2. Actin level was monitored to normalize protein loading. Quantitation of band density represents the level of IGFBP-5-associated expression relative to vector-transfected controls (1.0) normalized to the expression level of total Akt or ERK1/2. Results are representative of three independent experiments.