Ephrin A2 receptor targeting does not increase adenoviral pancreatic cancer transduction in vivo

Figure 1 EphA-2 expression in human cell lines. A: Analysis of EphA2R expression by Western blotting in human umbilical vein endothelial cells (Hu), human pancreatic cancer cell lines BxPc-3 (Bx), Hs766-T (Hs), Capan-1 (Ca), MIA PaCa-2 (Mia), p6.3, p10.5 and human fibroblasts (Fi). EphA2R was detected using a monoclonal antibody and detection of actin levels was performed as a loading control; B: Immunolocalization of EphA2R in human pancreatic cancer cell lines BxPc-3 (left top), Capan-1 (right top), Hs766-T (bottom left), MiaPaca-2 (bottom right). Cells were fixated with methanol, acetone, and water, and a directed monoclonal antibody was used to detect EphA2R using a goat anti-mouse labeled with PO to perform DAB detection. Magnification (× 600), except for MiaPaca-2 (× 400).