Higher CO2-insufflation pressure inhibits the expression of adhesion molecules and the invasion potential of colon cancer cells

Figure 5 Results of invasion assay. A: SW1116, 0 h after exposure; B: SW1116, 72 h after exposure; C: Lovo, 0 h after exposure; D: Lovo, 72 h after exposure. A: ¹At 0 h after exposure to 15 mmHg CO₂ insufflation, invasive cells decreased significantly compared to the cells prior to exposure (P = 0.019); ²At 0 h after exposure, invasion of the 6 mmHg or the 15 mmHg group was significantly different from the other pressure groups (P < 0.05). B: The invasion fluorescence intensities of SW1116 before and 72 h after exposure to CO₂ insufflation were not significantly different (P > 0.05). Seventy two hours after the CO₂ insufflation, there were no significant differences between the various insufflation pressure groups (P > 0.05); C: ¹At 0 h after exposure to 15 mmHg, 12 mmHg or 6 mmHg CO₂ insufflation, invasive cells of Lovo decreased significantly compared to the cells prior to exposure (P = 0.008, P = 0.0045, P = 0.043); ²At 0 h after exposure, cell invasion at 6 mmHg was significantly different from the other pressure groups (P < 0.01); ³At 0 h after exposure, cell invasion in the 12 mmHg group was significantly less than the 9 mmHg group (P < 0.05); ⁴At 0 h after exposure, cell invasion in the 15 mmHg group was significantly less than the 6 and 9 mmHg groups (P < 0.01); D: The invasion fluorescence intensities of Lovo before and 72 h after exposure to CO₂ insufflation were not significantly different (P > 0.05). Seventy two hours after CO₂ insufflation, there were no significant differences between the various insufflation pressure groups (P > 0.05).