Infant intestinal Enterococcus faecalis down-regulates inflammatory responses in human intestinal cell lines

Figure 2 Different cytokines expression in HCT116 cells. RT-PCR analysis was used to determine IL-8 (A) and TGF-β (B) expression. 10⁷ and 10⁸ cfu/mL E. faecalis (EC1, EC3, EC15, EC16), LP33 and T6 were treated with HCT116, mRNA expression of the cytokines were analyzed as described in Materials and Methods. Quantitative densitometry was carried out on EtBr-stained gels and the results from three independent experiments were compiled to produce the data shown (mean ± SD). The gel densities were normalized against β-actin.